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The field of transcriptome analysis using RNA sequencing platforms has developed rapidly in the past decade14.

At a practical level, the BFAST performance tests presented here demonstrate various modes of accuracy of alignment for both simulated data and real-world datasets from both the Illumina and ABI SOLiD sequencing platforms.

The constructed RNA-seq libraries were subjected to single end sequencing using Applied Biosystems SOLiD sequencing platforms.

The applications of the NGS sequencing platforms are endless [ 15- 18].

By first converting short reads into pseudo-Sanger sequences, we show that whole genome assembly using NGS sequencing platforms can be done efficiently.

To complete the genome, genomic DNA from L. santarosai serovar Shermani was sequenced and used to generate 454-FLX pyreadsuencing reads from 454-FLX pyrosequencing and SMRT sequencing platforms.

Library generation for Life Technologies' sequencing platforms (SOLiD and Ion Torrent) employs hybridisation to duplex adaptors with degenerate overhangs prior to ligation with trRnl2.

Therefore, in previous studies, RAD-seq has generally been performed using Illumina sequencing platforms, such as the Genome Analyzer IIx (GAIIx) and HiSeq series [ 1- 6].

The rapid evolution of DNA sequencing platforms has had a dramatic, beneficial impact on the study of microbial ecology and population genetics.

First, we generated single-end reads using Roche 454 pyrosequencing platform [ 24] and mate-pair reads with 3-4 Kb and 6-8 Kb insert fragment sizes, using ABI 3730 and MegaBACE 1000 Sanger sequencing platforms [ 25], respectively.

While global genomic re-sequencing remains overambitious given the large size of the wheat genome [ 35] along with the high content of repeat sequences (>80 %) [ 36], current DNA sequencing platforms are well capable of uncovering sequence variation in the transcriptome.

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