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A direct demonstration that these steady-state levels of GDNF are neuroprotective requires accurate titration experiments with viral vectors, and is technically difficult.
The only exception was Huh7-6 cells as this cell line does not support HCV infection sufficiently to be used for accurate titration analysis.
Since accurate titration of antibody levels is not possible with the IDEXX Mhyo ELISA, an additional titration step was performed to enable more accurate estimation of endpoint titres.
We attempted to perform this analytical approach, with an automatic titrator, which allows for precise readings and accurate titration of samples of small volume.
BJAB do not respond to partial MCL1 knockdown (not shown) and are therefore suitable for accurate titration of these vectors.
Unfortunately, there are numerous inherent limitations of existing therapies that include target nonselectivity, variable onset and offset of pharmacodynamic effects, a narrow efficacy-safety profile, and the absence of a safe and reliable platform for either accurate titration, based on existing patient-specific, disease-specific, and clinical conditions, or active reversibility.
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PEEP chosen by the PEEP titration procedure (titrated PEEP); PEEP set at 4 cm of H2O (PEEP4).
Accurate titrations require clean, precise, professional-grade chemical equipment.
In lab CPAP titration was performed with the AutoSet (ResMed Abingdon UK) auto-titrating CPAP device with full nocturnal polysomnography.
Two kinds of ELISA (screening ELISA, antigen titration ELISA) were performed as described before [ 74].
The NMR titration results were displayed in Figs 4d and S3.
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