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Specifically, HT-AC inhibited t-BHP-induced OPA1 cleavage and mitochondrial morphology changes, accompanied by improvement on mitochondrial oxygen consumption capacity, ATP productive potential and activities of mitochondrial complex I, II and V.
Similar(59)
(c) ATP production.
More ATP production was observed.
For kinases, allosteric mechanisms described to date include alteration of protein kinase conformation blocking productive ATP binding which appear 'ATP competitive' or blocking kinase activation by conformational changes that are 'ATP non-competitive'.
The PCH-2W221A pore-loop mutant was also not stimulated by MAD-2 + CMT-1, supporting the idea that this mutant uncouples ATP hydrolysis from productive substrate engagement.
However, the kinetic processivity (defined as the number of ATPs hydrolyzed per productive microtubule encounter) in these mutants is significantly less than wild type.
Put another way, the gating mechanism responsible for processivity in the 2-HB state does not require inter-head tension, but rather occurs through a modulation of the rates of biochemical processes occurring at the front head, including ATP hydrolysis, and possibly productive ATP binding (Clancy et al., 2011).
In addition, Mfd rescues arrested or backtracked transcription elongation complexes by promoting forward translocation of RNA polymerase in ATP dependent manner leading to productive elongation [13].
Binding of tRNAGlu induces conformational changes in GluRS that stimulates the binding of L-glutamic acid leading to the productive binding of ATP [ 16, 34, 35].
In vitro studies have demonstrated that these agents increase the rate of effective myosin cross-bridge formation, increasing the duration and amount of myocyte contraction, and inhibit non-productive consumption of ATP, potentially improving myocyte energy utilization, with no effect on intracellular calcium or cAMP.
A single metal is sufficient to interact with the enzyme through the formation of a productive MnATP-enzyme complex, while free ATP inhibits activity.
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