The ATP product provides the energy for the luciferase-catalyzed conversion of D-luciferin to oxyluciferin, with a concomitant release of photons.
In most biological reactions in which ATP is a product, it is likely to be synthesized from ADP, and consequently, ADP will be present as a substrate in assay solutions, potentially at a much higher concentration than the ATP product.
ATPs product doesn't have a huge storage capacity, so I'm not sure what you'd use it for, but the idea has promise.
We used fluorescent microspheres for PSF determination (175-nm diameter, 540-nm excitation, 560-nm emission; PS-Speck, product no. P-7220), fluorescent microspheres (actual size reported by Molecular Probes of 24 nm, nominal size of 20 nm; FluoSpheres, product no. F-8782), Alexa Fluor 488 (Alexa 488, product no. A-20000), and Alexa Fluor 647-ATP (Alexa-ATP, product no. A-22362).
As an example, the difference in free energy of substrate and product in an ATP-producing pathway limits the allocation of energy into ATP production versus driving the pathway, which may explain the trade-off between microbial growth rate and yield (Pfeiffer et al. 2001).
Its sensitivity lies in the micromolar range, and its ability to monitor ATP production or consumption was demonstrated with steady-state kinetic assays to measure time courses, in which ATP is a product or substrate, respectively.
We further validated our results by modeling the mycobacterial CoaE and studying its interactions with its substrates, DCoA and ATP; its product, CoA and the intrinsic metabolic regulator, CTP.
This might be in part because the ADP product of ATP hydrolysis is released by a much slower rate than Ser/Thr residues.
To determine the transitions of the actomyosin crossbridge cycle altered by reduced blood flow, we examined the impact of the ATP hydrolysis products ADP and Pi on steady state and transient force production of fibers prepared from an in vivo ischemia – reperfusion model [11].
In some bacteria, PEP is formed from pyruvate by the utilization of two of the high-energy bonds of ATP; the products include, in addition to PEP, AMP and inorganic phosphate [56].
NAD+, NADH, ADP, and ATP were products of Oriental Yeast Co. Ltd. (Osaka, Japan).
