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Two other auxiliary reactions were added to account for the ATP needs for cell maintenance [43] and protein turn over [44] (Table S1, ATP maintenance and Protein turnover reactions).
growth-associated ATP maintenance.
The generated ATP can be divided into two forms: non-growth-associated ATP maintenance (NGAM) and growth-associated ATP maintenance (GAM).
40643_2017_152_MOESM3_ESM.docx Additional file 3. Non-growth-associated ATP maintenance (NGAM) requirement.
On the other hand, ATP maintenance may also increase the maximal coupling yield for some metabolites.
This also holds true for most metabolites under anaerobic conditions but consideration of ATP maintenance requirements implies infeasibility of coupling for certain compounds.
Similar(13)
Mitochondrial-bound hexokinase (m-HKI) regulates important cellular processes in neurons, including ATP synthesis, maintenance of glutathione levels and neurite outgrowth [6], [7], [30].
Additionally, ATP for maintenance was included in the model and considered as an external metabolite.
This phenomenon has been observed previously in defined media, suggesting that the glucose feed is insufficient to provide ATP for maintenance and glycerol production [ 21].
CEF calculations were performed under a MATLAB 7.0 environment, and they were based on the efficiencies of calculated EFMs in terms of the chosen cellular objectives: production of biomass itself and ATP for maintenance.
In this state MTB requires NAD and ATP synthesis, and maintenance of the proton-motive force [16], [17], indicating that the bacteria remain metabolically active despite halted replication.
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