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Cell viability/death was analyzed by quantification of ATP levels and microscopical evaluation of propidium iodide (PI) uptake.
For the evaluation of ATP content under strict glycolytic conditions, the cells (3 × 10) in each well were incubated for 1 h at 37 °C in the presence of rotenone (1 μmol/l), a specific inhibitor of complex I, and antimycin A (1 μmol/l), which is a specific inhibitor of complex III, washed with PBS twice and suspended in PBS.
ATP = adenosine triphosphate; ENHANCE = Extended Evaluation of Recombinant Human Activated Protein C; iNOS = inducible form of nitric oxide synthase; LPS = lipopolysaccharide; NO = nitric oxide; PROWESS = Recombinant Human Activated Protein C Worldwide Evaluation in Severe Sepsis; RBC = red blood cell; SNO = S-nitrosothiol.
Therefore our results provide rationale for the clinical evaluation of ATP-competitive inhibitors of mTOR in colon cancer patients.
ATP and microbiologic sampling were performed immediately after performing FM and visual inspection evaluations, and each was performed in a non-overlapping area immediately adjacent to the FM.
ATP utilization and ATP synthesis.
ATP efflux.
ATP -- "adenosine triphosphate".
ATP is a nucleotide.
ATP is dead!
ATP is indie rock at its best.
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