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Citrex H4™ device was calibrated before each experiment and set in ATP condition.
For each case, five designs under the ATP condition are considered: the GIA-ATP, PMMSE-ATP, PMMI-ATP, PMBER-ATP, and PSVD-ATP.
The PMMSE-IAP has the smallest sum capacity because its transmitted power is less than that used by other designs under the ATP condition.
Movies were then recorded for the zero ATP condition.
The potential confounding factors besides genetic effect could include cell growth rate, EBV transformation and ATP condition.
In F – I, ***p<0.001; **p<0.01; *p<0.05, compared to the ATP condition for the same parameter (Mann–Whitney non-parametric t test).
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Unwinding rates of single XPD (magenta circles) and multiple XPDs (green squares) under different ATP conditions: (N = 3 9 traces at each ATP concentration; 15 800 μM).
Thus, Lis1 slows the microtubule off-rate of dynein in ATP conditions.
The serine/threonine kinase LKB1 activates AMPK under low ATP conditions [129].
(i) Dynein monomers release from microtubules in ATP conditions in the absence of Lis1.
33 Under saturating ATP conditions the de novo mutations (T99M, R216C and E253K) showed no motility (Fig. 5, Table S2, Video S1).
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