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Representative pictures of ATC specimens are shown in Fig. 6.
We detected CD133 expression in 8 out of 10 (80%) ATC specimens.
Our immunohistochemical studies of clinical ATC specimens clearly showed that CD133 is expressed on tumor cells but not on neighboring normal thyroid cells.
Nevertheless, our preliminary data with immunohistochemical analysis in 5 ATC specimens showed variable positivity for CD133 in all the samples analyzed (personal observations, not published).
We found alterations of the epigenetic machinery in all 4 ATC specimens with genome sequence data.
The expression of some of the intended targets of these drugs by our ATC specimens, and the 58 pairs of PTC and normal thyroid tissues, are depicted in the Additional file 2: Figures S2 and S3.
Similar(53)
Immunohistochemistry was performed on 10 archival routinely processed formalin-fixed, paraffin-embedded ATC tissue specimens.
24 Data on aspirin (ATC: B01AC06), clopidogrel (ATC: B01AC04), statins (ATC: C10AA), β-blockers (ATC: C07), ACE-inhibitors/angiotensin 2 receptor blockers (ATC: C09), furosemide (ATC: C03C), aldosterone antagonists (ATC: C03D) and antidepressants (ATC: N06A) were collected.
These results warrant further investigation on large numbers to definitely confirm CD133 expression in all ATC ex vivo specimens.
More importantly, immunohistochemical analysis of tissue specimens from ATC patients indicates that CD133 is highly expressed on tumor cells but not on neighboring normal thyroid cells.
Each test was performed under fully reversed cyclic quasi-static loading in the elastic and inelastic response zones of the specimens, in compliance with the ATC-24 (1992) test protocol.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com