Even the most trivial deviation from a technical protocol culturing cells, quantifying proteins, detecting RNA can make an experiment difficult to replicate.
When all 20 metaphases examined were normal or when at least 18 metaphases were normal with one or two demonstrating a non-clonal, simple chromosome loss (which is most likely a technical artifact), the cell line was concluded to have normal karyotype.
Once considered a technical snag in cell fractionation techniques, the alleged ER vesicle contaminants that invariably appeared in the mitochondrial fraction have been re-identified as membranous structures derived from the MAM the interface between mitochondria and the ER.
From a technical aspect, the cell identification would be an impressive feat.
ALDH activity is detected by a fluorescent green emission, a technical limitation for cell sorting in organs (such as the liver) rich in endogenous fluorophores (flavins, NADPH) that autofluoresce in the green wavelength (∼480 580 nm).
The organizational-technical cell (3) comprises a company's core technical competencies.
In the table above, the individual-technical cell (1) represents a person's functional competence, such as technical expertise in marketing, finance, or manufacturing.
1) The failure to produce full iPS-like cells from fly cell lines is potentially interesting but could reflect a technical problem with the cell line used.
There's huge excitement over this type of treatment, called CAR-T(for Chimeric Antigen Receptor T-Cell, a technical description of the modifications made to cells).
Such lack of precipitation in the ER lumen could be because of a technical reason that the cell selected for EM examination was not transfected.
More and more, telcos are concerning themselves not just with whether things work from a technical standpoint ("Does my cell phone have a signal?"), but with the emotions of the customer ("Am I having fun watching this video – or feeling impatient and frustrated?").
