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A TCF reporter assay showed that there is no aberrant Wnt signalling present.
The inhibition of β-catenin/TCF transcriptional activation by sulindac sulphide was monitored by transfecting a TCF reporter (pTOPflash) or, as a control, a construct containing scrambled TCF binding sites (pFOPflash) in both CRC cell lines.
TOPglow features the gene for firefly luciferase, under control of a TCF promoter; FOPglow features the same reporter gene, but downstream of a mutated TCF-binding site.
Using a TCF reporter assay, we found that these FHC epithelial cells have no detectable TCF-mediated transcription.
Officials from JPMorgan and Capital One declined to comment; a TCF spokeswoman did not return a phone call seeking comment.
In comparison to a TCF 20 under the same test conditions with conventional gas bearing turbines this is an improvement of 52%%.
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For this purpose we transfected N2a-m cells with a TCF-luciferase reporter (TOPFlash) in combination with an EGFP reporter.
Transcription of a TCF-reporter in a transgenic mouse model is enhanced by estradiol in a similar fashion to that produced by Wnt3a.
In neuronal cells, estradiol induced the formation of a TCF-DNA complex that was impaired by the presence of antibodies against LEF-1.
With this aim, we selected a neuronal cell line easily amenable to transfection with a TCF-reporter: the N2a-m cell line which expresses the α and β ER receptors and accumulates nuclear ER when treated with estradiol.
Consistently, cotransfection of mouse Wnt3a and human FZC18 or V3Nter with a TCF-responsive reporter in the HEK293T and in the Huh-7 cell lines showed that FZC18 and V3Nter suppressed Wnt3a-induced CRT by more than 80% (Figure 6C).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com