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A TBP modifier to the D2EHPA was also tested to determine the effect on process efficiency.
A TBP stock solution of unknown concentration was dried by rotary evaporation in the dark, immediately dissolved in dimethylsuloxide (DMSO), and stored at 4°C until use in bioassays.
Tests conducted with a TBP apparatus using mixtures of pure components showed differences of mainly less than 3% between the weight % of the separated components and the original weight % of the pure components in the mixture.
The expression profile of a TBP mutant (F182V; [48]) that is unable to bind NC2 shows similar behavior.
Therefore, previous analyses have revealed not only how Mot1 binds TBP, but also that Mot1 functions as a TBP chaperone.
In our low-pass sequence analysis, the presence of a TBP homolog was not observed in the newly sequenced halophiles.
Similar(47)
To test the specificity of the immuno-precipitations in Figure 1B, we present a mock IP control in which the different proteins involved in this study were examined (CENP-A, TBP, CENP-C and RNAPII).
The MADF domain directs sequence specific DNA binding to a site consisting of multiple trinucleotide repeats, while the BESS domain directs a variety of protein protein interactions, including interactions with itself, with Dorsal, and with a TBP-associated factor.
In addition, USP10 is identified as a TBP-specific deubiquitinase and counters TBP degradation and myocyte differentiation.
We surveyed EF1-α, β-actin, TATA box Binding protein a (TBP-a) and 18S rRNA as potential internal control genes for RT-PCR (data not shown).
Given the tight temporal control of TBP-dependent mRNA degradation, we hypothesised that those miR-430 target mRNAs are degraded in a TBP-dependent manner, which are eliminated at a 'medium' rate during late blastulation/early gastrulation.
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