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At least 2 of these object stimuli (Fig. 5 C ) activated 4 (Fig. 5 A, spots A D) and 5 activity spots (Fig. 5 B, spots E I) in hemispheres H1 and H3, respectively.
We deliberately create tests that have some minor issues lurking within them, purely to see if the candidate (a) spots them and (b) is willing to fix them.
Sure, you can put a pole dancer in spandex shorts and a spots bra, but when she is performing a vertical over-split, the word 'conservative' does not come to mind.
To more precisely identify the Bves-Bves interaction region in the C-terminus, a SPOTs blot membrane was generated by SIGMA-GENOSIS (Woodland, TX).
a spots are defined in Figure 12e.
a Spots based on MRSA252 ORFs (75% of all spots on the array).
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Blowup to the right represents a single slice of the boxed region showing that CENP-B is located in between the CENP-A spots of sister centromeres.
Whereas punctate CENP-A spots can be seen under both conditions, when RNAPII was blocked, the intensity of the CENP-A signal was decreased by ∼50% in cells.
Intriguingly, we sometimes observed an elongated CENP-ACnp1 signal flanked by two Mis12-GFP or Nuf2-GFP spots (in no case were two CENP-A spots seen flanking an elongated GFP signal).
Blowups show the chromosome containing the integrated Lac-array (A ); the Y-chromosome (outline indicated; CENP-B negative) as well as an autosome (CENP-B positive) (C ); and the neocentric chromosome 4, containing 2 pairs of ACA spots (staining both CENP-A and CENP-B), but only 1 pair of CENP-A spots (E ).
But Nagoski's advice regarding g-spots -- and a-spots and p-spots -- alarms me even more.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com