Sentence examples for A site directed from inspiring English sources

A site directed mutagenesis kit (Promega) was used.

Starcom clients will be able to help shape the sites that are still in development, including a political one for MSNBC.com and a site directed at Latinos.

Generation of coding sequences of AtMTM1 with mutations in the active site was carried out by a site directed mutagenesis approach using an overlap extension PCR approach [42].

To investigate the contribution of these conserved amino acid residues to the L. major HemG activity a site directed mutagenesis approach was pursued.

In this instance, a site directed mutation could be utilised to dictate the location of the specific residue and, hence, control the orientation of the molecule (e.g additional Cys residue for thiol immobilisation [ 20]).

The internal XbaI site of the gne gene was eliminated by a site-directed mutagenesis (QuickChange Multi Site-Directed Mutagenesis Kit, Stratagene, La Jolla, CA) using GE3 (5'-CTGAACTAGCACATC GAGAGTTAGGTTGGTATGC-3') primer.

All plasmid constructs were generated using PCR-based strategies using appropriate primers; point mutants were generated using a site-directed mutagenesis kit (Chen and Mallampalli, 2009).

We are currently studying the feasibility of targeting chromosomal genes using this approach, either by directly inducing mutations in the target gene or by stimulating recombination in a site-directed manner.

Point mutations in MC4R were introduced with a site-directed mutagenesis kit (QuikChange, Stratagene, La Jolla, CA) and sequenced to confirm the substitutions.

A pGL3 construct containing DNMT3A 3′UTR with point mutations in seed sequence was synthesised using a site-directed mutagenesis kit (Stratagene, La Jolla, CA, USA), according to the manufacturer's instructions.

Valentini, G. et al. The allosteric regulation of pyruvate kinase: a site-directed mutagenesis study.