Sentence examples for A overhang from inspiring English sources

We initially used the Promega enzyme to ensure the presence of an A overhang for downstream cloning.

The fourth base of the index was a thymidine used for ligation to the 3' A overhang DNA fragments.

The final 'T' at the fourth base anneals with the 'A' overhang from the end-repaired DNA sample for ligation of ChIP or other DNA fragments.

The addition of a 3' A overhang was described above.

Subsequently, a single nucleotide (A) overhang was added to the digested fragments.

An "A" overhang was added to the ends of the fragments.

For example, TA cloning uses regular Taq DNA polymerase to add a single 3'-A overhang to the ends of the PCR product.

One was that it allowed for the A- overhang needed for cloning, and also it was the common system in use at the time of data collection (prior to the common availability of the Roche enzyme system).

To express in mammalian cell culture, npr-17 cDNA was amplified from N2 cDNA using MyTaq DNA polymerase (Bioline, Taunton, MA) to generate PCR products with a 5′ Kozak consensus sequence and 3′-A overhang.

It's of a Native American bouldering down an overhang with a big bag of wood on his back.

The A-overhang added PCR product was purified using MinElute Gel purification kit (Qiagen, Germany).