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A MGMT activity assay was performed on each sample as previously described (Watson and Margison, 1999).
They have a MGMT vibe, and the singer is a bit weird: they did a showcase where he started off by doing standup comedy, and people didn't understand the humour, so he got pissed off and didn't do the gig.
We anticipate that further refinements in a MGMT methylation test for use in clinical decision-making might include a test similar to our MS-MLPA 3R classification method.
Interestingly, non-CIMP anaplastic gliomas showed a MGMT promoter methylation frequency similar to glioblastoma.
The percentages of patients under 50 years, with an MMSE above 27 or with a unifocal tumour location are higher in patients with a MGMT status.
Patients from the TCGA-GBM dataset (n = 239) with a MGMT methylated glioblastoma had a more favorable outcome (p = 0.047, log-rank test, Fig. 4).
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Lately, there's a … I can't remember the name of it, but an MGMT song that's killing me right now.
89 In order to overcome MGMT-induced TMZ resistance, some trials have assessed the efficacy of combination regimens with TMZ and O-6-benzylguanine (an MGMT inhibitor).
Thus, p53 is a negative regulator of MGMT gene expression which can create an MGMT-depleted state in human tumours (Srivenugopal et al, 2001).
This sensitizing effect is detectable when leukaemia cells show resistance mechanisms based on a MGMT-proficient phenotype.
To counteract the gene, physicians sometimes add an MGMT-blocking drug, benzylguanine, to the chemotherapy regimen to make the cancer cells easier to kill.
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