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A GUS DNA fragment was amplified via PCR with the primer pair, GUS (Additional file 1: Table S1), and was used as probe.
A GUS histochemical assay showed that only the full-length Gacab promoter was light inducible and expressed in a tissue-specific manner.
A GUS staining experiment by razorblade section was used to further investigate the exact location of GUS gene expression.
A GUS assay confirmed it was only expressed in the roots, especially in a small region behind the AR cap.
(A ) GUS staining of transcriptional reporters for cell cycle genes CDKB1 1 and CDC6 (rows) in WT Col (left column), amiRBR (middle), and FAMALGK plants (right column).
A GUS reporter gene approach in Arabidopsis showed that activity of the VvMYB60 promoter was restricted to stomatal guard cells and was down-regulated by ABA.
This low level of GUS activity was confirmed using a GUS quantitative assay.
To analyze the promoter activity of UGT73C6 in different tissues and developmental stages a GUS reporter lines was constructed in which the GUS gene was expressed under control of the UGT73C6 promoter (UGT73C6 pro GUS).
To construct the ß-glucuronidase (GUS) reporter under the control of CRP4 promoter, a GUS coding sequence (plasmid pRT102) [ 55] was inserted downstream of the CRP4 promoter and introduced into pPZP221 binary vector [ 53].
To examine this interesting speculation, we conducted compartment plate assays using an A. thaliana Col-0 transgenic line stably expressing a GUS reporter gene fusion to the auxin responsive promoter DR5 (DR5::GUS).
At Lever House in Midtown, the bar serves a Gus Meyer-lemonade with lemon vodka and mint.
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