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In order to exclude that the reduction of cell viability related to any off-target effects instead of CSB suppression, we performed rescue experiments with a CSB cDNA that was resistant to ASO.
The ability of a CSB to achieve super-resolution has been also discussed in recent works.
After obtaining the vertical shear buckling strength of a CSB through finite element model, the shear buckling coefficient k can be obtained through inverse analysis.
The ability of a CSB to resolve two sources below the Rayleigh limit, even using a single snapshot, is strictly related to the fundamental Theorem 1.3 in [24].
For example, in [16], the authors investigate the super-resolution property for a CSB for the single and the multi-snapshot scenarios.
Instead, providing a sufficient level of SNR and a suitable number of grid points, a CSB is able to resolve sources that are in the same Rayleigh resolution cell.
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Control (Scrambled non-specific) and CSB-specific shRNA vectors were used to create a CSB-proficient cell line (sh-K) and a CSB-suppressed cell line (sh-CSB), respectively.
This interaction was also observed in CSB-deficient patient cells, indicative of a CSB-independent binding of UVSSA to TC-NER complexes.
In UVSS-A cells, stalled RNA Pol IIo can still be ubiquitinated by a CSB- and CSA-dependent pathway, but cannot be deubiquitinated due to the absence of the UVSSA-USP7 complex [ 8], so that transcription resumption does not occur and the cells are RRS deficient.
MB-A, MS, CSB and PAA designed the study.
Shear buckling behaviors of web-post in a Castellated Steel Beam (CSB) with hexagonal web openings under vertical shear were investigated using finite element method.
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