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Total membranes were extracted using a CHAPS containing buffer, and subjected to tandem affinity purification.
After removal of the chloroform under vacuum over night the lipid was first resuspended in water at 10 mg/ml and then partially dissolved in CHAPS by incubating for 1 h at room temperature with a CHAPS to lipid ratio of 3 1 and a lipid concentration of 7.5 mg/ml.
Adhesion of WT neurosphere-derived cells plated on a CHAPS protein extract of embryonic forebrain showed a difference depending on the genotype of the substrate/extract (Fig. 4 B ). Whereas an average number of 40 cells adhered on noncoated glass coverslips (control), the adherence on 10 μg/μL embryonic WT brain extract was decreased by approximately 35%.
Similar(57)
He does remember a chaps-wearing woman with a British accent hopping on the filly for a walk around the backstretch and liking her enough to take her home; but she didn't want the filly's registration papers.
Philby was a chap.
Does a chap ask for much more?
What sort of a chap is he?
I say, give a chap a brandy and soda".
There was a chap with his hair on fire.
A chap can be forgiven a lot for that.
"One day a chap came wandering in, very obviously deranged.
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Justyna Jupowicz-Kozak
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