Two different challenge protocols were used: once weekly airway challenges for 2 weeks (short term) or for 8 weeks (long term).
Mice were fed with a methionine choline-deficient (MCD) diet (MP Biomedicals) for 2 weeks (short term) or 8 weeks (long term).
The follow-up measurements will take place by a blinded research assistant immediately after the 8 weeks treatment period and after 26 weeks (long term follow-up).
6 9 In patients with persistent aneurysms beyond 6 weeks, long-term cardiovascular follow-up into adult life is required.
We compared our observations with those of Quinet et al. (2012), who studied gene expression in 2-week Kong Pao (indica rice) plants in response to 125 mg L−1 FeSO4 for 3 days (short-term) and 3 weeks (long-term).
Mice were exposed daily to HDM for either 10 consecutive days (short-term protocol) or 5 consecutive days a week followed by 2 days of rest for a total of 1, 2, 3, 5, 7, 10, 14 and 20 weeks (long-term protocol).
Short term outcomes typically 4 to 8 weeks, long-term outcomes typically 6 to 12 months.
Both indirect and direct EDC effects were observed at environmentally relevant concentrations with exposure durations between 4 h and 7 days (short-term) or 6 to 12 weeks (long-term).
With rapidly progressive disease models, such as rat MIA, often only evaluated for 3 to 4 weeks, long-term side effects or toxicity of therapy are unlikely to become apparent.
The primary research hypothesis is that survivors of a critical illness who participate in the physical rehabilitation programme will have better physical function, as measured by a difference of 10 points on the SF-36 PF subscale, when compared with those who receive usual care at eight weeks after hospital discharge (short-term effect), which will persist at 26 weeks (long-term effect).
In this study, the two types of cord blood human HSC-engrafted BALB/c Rag2−/−; Il2rg−/− mice showed similar levels and differentiation ability, giving about 50% human-circulating leukocytes at 5 weeks, long-term persistence of human cells in lymphoid organs and production of human antibodies.
