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A Box Behnken design was used to investigate the effect of amount of sodium bicarbonate (X1), pore former HPMC (X2) and glyceryl behenate (X3) on percent drug release at 1st hour (Q1), 4th hour (Q4), 8th hour (Q8) and floating lag time (min).
The cefoperazone and sulbactam concentrations in CSF were highest at the 4th hour (39.22±75.55 mg/L and 6.24±8.35 mg/L, respectively) and then decreased.
The extract at 200 mg was able to decrease the BGL significantly (60%; p < 0.05) on the 4th hour compared to initial value (Table 1).
The maximum level of IL-1 β release was observed within the 3rd hour and 4th hour, when 60-day cryopreserved (both in glycerol and DMSO) lymphocytes were stimulated by LPS and LTA– 5(d)).
Inter-group analysis revealed that O. integrifolia at 100 mg/kg and 200 mg/kg reduced the 4th hour fasting blood BGL significantly (p < 0.001) compared to the control group.
Cultures transferred to 12°C and 25°C were sampled every 4th hour for 52 hours, two time points in light followed by 12 time points in DD, as indicated in each figure.
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