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Thus, the cells were treated with 0.2 U/mL of IFNγ and small molecules for a total of 48 hrs before fixation.
Plates were incubated at 22°C for 48 hrs before counting the cfu on plates.
Briefly, an initial preparatory surgery was performed 48 hrs before the first neural recording session.
These cells were then grown for another 48 hrs before selection with 2.5 µg/ml puromycin (Calbiochem).
Administration of Il12 and CpG DNA intranasally 16 and 48 hrs before RWE challenge, respectively, upregulated Gbp1, Iigp and Socs1 more than RWE challenge alone (Figure 5A).
Tissue samples were dried at 60°C for 48 hrs before being ground to a powder using a Retsch® ball mill (Haan, Germany).
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Venous blood was obtained 2 hr before, immediately after, and 20 hr after each exposure.
Cells were cultured for 48 hr before infection was assessed following intracellular p24 staining.
Cells were rested for 48 hr before experimentation.
Transfected cells were incubated for another 48 hr before immunofluorescence analysis.
The J7-21 cells were plated for 24 hrs before priming.
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