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For inoculation 4 week old Arabidopsis plants were used.
Results are based on single leaf analysis of 4 week old plants.
Leaves (3 10 mm) from 3 to 4 week old shoots were cut at the base.
RNA was isolated from 4 week old plants using TRIZOL® reagent (Invitrogen) according to manufacturer's instructions.
Lesions area (%; mean ± SE) were measured from 4 week old LD soil-grown plants.
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In brief, Arabidopsis protoplasts were isolated from the leaves of 3-4 week old plants.
Leaf tissue was collected from 3 4 week old Brachypodium plants.
All mice used were 4 week-old females.
Approximately 4 week-old plants were used for crossing.
(A ) Expression of genes upregulated in 4 week-old sgn3 leaves.
Detached leaves from 4 week-old plants were dark-adapted 20 min prior to measurements.
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