Sentence examples for 3rd step from inspiring English sources
There are novel folds, such as the folds of methenyl-H4MPT cyclohydrolase (Mch, 3rd step) and N 5 -N(10)-metheN 5 -NMPT dehydrogenase (Mtd, 4th step in the pathway) enzymes, which emerged in response to demand for new/specific function.
In order to establish a more efficient method for VSEL purification from UCB, we employed a three-step isolation strategy based on removal of erythrocytes by hypotonic lysis (1st step), followed by immunomagnetic separation of CD133+ cells (2nd step), followed by FACS-based isolation of CD133+Lin−CD45− cells (3rd step).
The 3rd step (2006) was a specific strategy to quickly control the spread of emerging MDR (vancomycin resistant enterococci, VRE; carbapenemase producing enterobacteria, CPE).
The MtLPMO9A preparation thus obtained (partially purified fraction SEC-I; Additional file 1) was further subjected (3rd step) to a Resource Q column (30 × 16 mm internal diameter, GE Healthcare), pre-equilibrated in 20 mM potassium phosphate buffer (pH 7.0).
Regarding the 3rd step, anti-CD4 antibody application suppressed CD4+ T cell accumulation and EAE-relapse but not somatosensory activations, sympathetic activations, or MHC class II+CD11b+ cell accumulation in the ventral vessels, suggesting that CD4+ T cell accumulation is a downstream event of sensory and sympathetic activation and of MHC class II+CD11b+ cell accumulation in the ventral vessels.
Now same with the 3rd step, cut as many 15 inch (38.1 cm) pieces of string.
After "Liam" comes the number 4 at the bottom: 4 - Step 1. 3 - Step 2. 2 - Step 3. 1 - Step 4. Garbage Truck - Step 5. Jeep Wrangler - Step 6.
The 264 step National Wallace monument.
3. Step into purposeful leadership.
