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WNV-NY99 (3rd passage in Vero cells of strain NY 385-99) [ 20], was kindly provided to OMRIX biopharmaceuticals by M.S. Diamond, Washington University, School of Medicine, St . Louis MO, USA.
Finally, these 100 µl were depleted one more time with ProteoPrep20 (3rd passage).
Late brain-inoculated mice died at ∼500 days, a survival time that neither changed on 3rd passage (497±18; 10/10 mice) nor differed significantly from that seen upon primary transmission of vCJD on homozygous mice (Table 1).
A virus stock containing approximately 10 median tissue culture infective dose (TCID50) per ml (3rd passage) was grown initially on Vero cells and used to develop and optimize the VNT.
Cytospins were prepared from melanocyte cultures at an early phase (first passage in culture, approximately 7 10-day-old culture), at 3rd passage in culture (3p, about three weeks after primary plating) and at late phase in the 7 10-day-oldcultures (generally five weeks into culture).
Robust expression was detected by WB from the ubiquitous EF1α promoter and negligible expression from the MeP promoter in the 1st, 2nd and 3rd passage (Figure 7A).
Similar(27)
· 1-5 passage Ruelle, Paris.
Le Passage is at 18 Passage de la Bonne-Graine.
(3) Passage of the American Board of Internal Medicine Institute for clinical evaluation, ECG certifying examination. .
Stage 3: passage through regional intermediaries/international traders.
Our results were consistent with earlier studies by Leung et al.[22] who found that ARPE-19 cells and RPE cells from primary cultures (2nd 3rd passage) only expressed IL-17RC mRNA, but definitely not IL-17RA mRNA.
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