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To investigate whether our combined dietary strategy could alleviate chronic epilepsy, we injected KA (20 mM, 0.05 µl) into the CA1 area of the hippocampus of 6 weeks old C57BL/6j males (Fig. 4A), and then waited for 3 weeks for the epileptic phenotype to develop before feeding the mice with SD, CD or KD for an additional 3 weeks period (Fig. 4B).
At the end of the 3 weeks period of daily immobilization, the weight of adrenal glands relative to body weight was significantly increased compared to controls reflecting enhanced activity of the hypothalamus-pituitary-adrenal axis in stressed animals (Fig. 5B).
Interestingly, this occurred despite Dom showing a 3 weeks-long hyperphagia while Sub being hyperphagic only in the last 2 weeks (Sub clearly ingested more kcal when compared to baseline throughout the 3 weeks period. Figure 6B).
The tablets were taken 2 hours before bedtime for a period of 3 weeks (period 1).
In a randomized, double-blind, crossover study, the subjects were treated for 3 weeks (period 1) with prolonged-release melatonin or placebo, followed by a one-week washout period, and then crossed over for another 3 weeks (period 2) of treatment with the other preparation.
During the 3 weeks period when mice were supplemented in their drinking water with 2 mg/ml doxycycline/5% sucrose, animals were kept in cyclic light (12 h light and 12 h dark; 60 lux) prior to all experimentation.
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Animals were killed over a 3 week period and the eyes examined for CNV by histopathology.
Following this initial 3 week period all populations were sieved.
The instruction started and lasted for a 16 weeks period.
A total of 14 treatments over a 8 weeks period Amitriptyline (n = 70).
Bacteria colonize nodule cells, differentiate into membrane enclosed bacteroids and a mature nitrogen-fixing nodule forms in 2 3 weeks period [ 5- 7].
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