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Real-time PCRs were done using primers and probes specific for U5, 2nd transfer and 2-LTR circles [59], [60], Serial dilutions of HIV-GFP or 2-LTR plasmid in genomic DNA were used to generate a standard curve in each plate.
Seven days following the 2nd transfer, mice were sacrificed and lung and spleen cells were enriched using a homogenizer.
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16) Transfer husband.
2. Transfer to a blender.
3. Transfer the pan to the stove.
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3. Transfer mixture to a large bowl.
Bradley is a 6-10 transfromfrom Kentucky.
3. Transfer mixture to baking dish.
3. Transfer sauce to a small pitcher.
2. Transfer rabbit to a platter.
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