Sentence examples for 2nd selection from inspiring English sources

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It should be noted that we obtained not only MIP but also peptide X12-2 with approximately the same frequency in the 2nd selection (Fig. 2B).

Six residues in MIP (Phe3, Tyr6, Trp7, Arg9, Leu10 and Met11) were replaced by Ala because these six sites were found to be retained in the peptides obtained from the 2nd selection (Fig. 2C).

We therefore increased the stringency of the selection process to enrich for specific peptide sequences during the 2nd selection by shortening the binding time from 2 h to 5 min and increasing the number of washes from 3 to 15.

Moreover, from the peptide sequences obtained from the 1st selection (Fig. 2A) as well as the previous reports [24], we postulated that three hydrophobic residues corresponding to Phe19, Trp23, and Leu26 of wild-type p53 as the optimal binding motif to bind to MDM2, and we fixed these residues in the 2nd selection.

We did not fully characterize the T191I NA mutation that emerged in our 2nd selection experiments, but based on observation that this amino acid is located far from the NA active site we expect that this mutation likely affects NA activity by reducing NA incorporation into virions, rather than directly altering enzymatic activity.

To improve the efficiency of the selection strategy, we performed a 2nd selection.

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Similar(47)

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1) Selection  .

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