Sentence examples for 2nd passage from inspiring English sources

For vaccine preparation, MARC-145 cell culture supernatants of 07 V063 (2nd passage on PAM + 2 passages on MARC-145), 08 V194 (2nd passage on PAM + 4 passages on MARC-145) and LV (2nd passage on PAM + 5 passages on MARC-145), were purified via ultracentrifugation as previously described by Vanhee et al. (2009) [ 25].

Briefly, serum samples were twofold serially diluted and an equal volume of a PRRSV 07 V063 (2nd passage on PAM + 2 passages on MARC-145) or 08 V194 (2nd passage on PAM + 4 passages on MARC-145) suspension (titer 2 × 10 TCID50/mL) was added to each dilution.

For challenge, macrophage-grown stocks were prepared of the isolates 07 V063 (2nd passage on PAM) and 08 V194 (5th passage on PAM).

The reason for this tendency is unclear but it had already been reported for ovine BSE transmitted to this model (296 d.p.i at first passage to 365 d.p.i at 2nd passage) [17].

To determine effects of long-term culture on DNA methylation patterns we harvested MSC of early passages (2nd passage; P2) and late passages (P8 P15) from eight donors.

L. donovani strain AG83 (MAOM/IN/1083/AG83) was used to infect 4- to 6-week old BALB/c mice with 2nd passage promastigotes or amastigotes (10 parasites/animal) by the intracardiac route.

· 1-5 passage Ruelle, Paris.

Le Passage is at 18 Passage de la Bonne-Graine.

(3) Passage of the American Board of Internal Medicine Institute for clinical evaluation, ECG certifying examination.  .

Stage 3: passage through regional intermediaries/international traders.

Progeny viruses from trial 1 (passage 7) and trial 2 (passage 6) were plaque purified.