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Importantly, the detection and imaging of IFN- γ protein in resting or activated macrophages has been elusive, and confocal immunofluorescence is the preferable technique for its cell-associated detection (see review ref. 26, pg. 288, 2nd paragraph).
This reasoning is also mentioned in Results/Discussion, part 1, 5th paragraph and part 3.1, 2nd paragraph.
Page 13 2nd paragraph: "profile-to-profile HMMER method" --> "profile-to-profile HMM method" Page 19 2nd last paragraph: "dissecthmmer" --> "dissectHMMEresponsers' response: The minor points have all been addressed in the revised manuscript.
Minor comments: a couple of typos at page 3: 2nd paragraph "of the functional dual topology H,K ATPase"; 4th paragraph "The APM showed very high basal( Ca2+….)".
Page 12 2nd paragraph: "for better or worst" --> worse?
Within the Results, paragraph 6 (2nd paragraph within Validity), the last sentence should now read as "The associations with trait anxiety and magnitude of changes in work were weaker".
I do not think it is tested, since it is not possible to do a phylogenetic tree with HslV, the alpha and the beta subunits, as you explained after, p. 25, 2nd paragraph.
However, we decided not to do this for several reasons, which are explained in the text with appropriate references (please, see also the Results/Discussion, part 3.1, 2nd paragraph).
Similar(3)
P.6 2nd paragraph: "There are two types of scalp hair, vellus/Ianugo and terminal hair.
P.5 2nd paragraph: "The first report of phycodnavirus isolations was from the prasinophyte Micromonas pusilla [ref 47].
P.3 2nd paragraph: "[Investigators] are now able to repeat the original calculation for the number of bacteria in the colon [ref 3].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com