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lacZ expression is used to measure long-term activation of neurons (i.e., in response to the first experience, days ago) whereas c-fos or zif268 is used to measure recent activity (for the 2nd experience).
This gives the impression that Dox is removed at the time of the 2nd experience when in fact it is removed much earlier, in the home cage situation.
For example, to what extent does the 2nd experience activate neurons due to emotional content (recalling a memory of being shocked) vs. due to the fact that it's simply a different spatial environment?
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