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DNA was extracted from frozen tissues by lysis buffer/proteinase K treatment and phenol-chloroform extraction as previously described (20 ), omitting the deparaffinization step.
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The agarose resin was washed five times with lysis buffer (adjusted to 150 mM NaCl, 0.09% Tween-20, omitting the PhosSTOP reagent).
Elution of the kinesin heterodimeric constructs from M2 agarose was achieved by applying one resin volume of 3xFLAG peptide at final 2 mg/ml in lysis buffer (adjusted to 250 mM NaCl, 1 mM DTT, 0.09% Tween-20, omitting ATP and PhosSTOP).
However, the overall residual means and standard deviations did not improve (omitting item 9: mean [SD], -0.498 [1.171]; omitting item 24: mean [SD], -0.494 [1.282]) and reliability decreased slightly (omitting item 9: 0.831; omitting item 24: 0.840).
Negative control procedures were performed to assess the specificity of the ISH signal: 1) omitting Dig-labeled probe; 2) omitting anti-Dig antibody; 3) omitting Dig-labeled probe and anti-Dig antibody.
3-5 ominted inumberingnumbering
(000 omitted in dollar figures) 3-Mo.
Cataloger supplied pagination p. 45-71 omits blanks.
(Voyage in reverse direction on Sept. 6 omits Santander).
(Seven-night sailing on Sept. 16 omits Quebec and Saguenay Fjord).
Carbon copy of the typescript listed in the Finding Aid under number 51. 508 leaves numbered 1-510 (76 missing, 207 repeated, 258 omitted, 348 repeated, 349 omitted, 465-66 omitted, 480a added, 501 repeated, 502 omitted).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com