Sentence examples for 2 supplemented from inspiring English sources

Low CP corn-soybean meal basal diets (14% for Exp. 1 and 11% for Exp. 2) supplemented with crystalline lysine, methionine, threonine, and tryptophan were formulated.

Dietary treatments were: (1) control diet (CON; n=25), not supplemented with RPN and (2) supplemented diet (RPN; n=25), supplemented with RPN (15 g/cow daily).

Diet 2, supplemented with olive and linseed oils was designed to provide moderate amount of linoleic (18 2n − 6) and linolenic (18 3n − 3) acids in the form of triglycerides.

The cell lines were cultured in Keratinocyte Basal Medium® 2, supplemented with SingleQuots® (Cambrex Bioscience; Walkersville, MD), as previously described [39].

Additional file 5: Verification of DAB visualized peroxidase location with H 2 O 2 supplemented TMB.

Primary HUVECs were obtained from Lonza (Basel, Switzerland) and cultured at 37°C in an atmosphere of 5% CO2 in endothelial cell growth medium 2 supplemented with endothelial cell growth medium 2 microvascular endothelial cell medium 2 SingleQuots (Lonza).

On day 3, T cell blasts were harvested and further expanded or transduced in IL-2 supplemented media.

Protoplasts in the centrifuge tube were mixed with 1 ml of stabilizing solution II consisting of 0.1 M sucrose, 0.1 M CaCl2 and 0.1 M Ca(NO3 2, supplemented with 1% clarified V-8 juice.

Medium was replaced with 2.5% EBM-2 supplemented with 1% ECGS.

Basal medium comprised EGM-2 supplemented with ascorbic acid, heparin and GA-1000 but without serum or growth factors.

Further, HAECs cultured with [PAGS∶heparin∶FGF-2] media was the only group that stimulated statistically longer tube formation than bolus FGF-2 supplemented media, outperforming fresh heparin-stabilized FGF-2, indictaing the potential of the polycation-based delivery system.