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Based on present and previous findings, we may speculate whether the 2 spiking types that appear here originate in different zones of this widespread neuron.
These included: 1) Replicated analysis of samples to test robustness of microarray data; 2) Spiking DNA of underrepresented species to a complex mixture followed by microarray analysis; 3) performing a direct comparison between qPCR and microarray data, and 4) performing a direct comparison between sequencing and microarray data.
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To discriminate G/A SNP, the SPIKE-01SNP marker consisted of two PCRs (PCR #1, SPIKE-01SNP-GF/R; PCR #2, SPIKE-01SNP-AF/R).
For cells that generated at least 2 spikes, the amplitude ratio of the 1st and the 2nd spikes in the train was reported as #20 'Spike accommodation'.
Proteins were incubated in ATPase buffer 2 spiked with hot ATP for 1 hour at 30°C.
104F spiking to 106F in the throes of legionnaires' disease.
When (z = 0) (Fig. 1A) spiking is much faster than when (z = 0.7) (Fig. 1C).
These suggest that Ca2+ spiking by salt stress triggers the SOS3-SOS2 mediated salt stress signaling pathways.
It has been well established that high salt stress rapidly leads to cytosolic Ca2+ spiking.
At the same time, Myc factors secreted from AM fungi induce symbiotic Ca2+ spiking.
Using fluo-4 AM dye, we found spontaneous Ca2+ spikes in 18% of rat SCN cells in acute brain slices, but the Ca2+ spiking frequencies showed no day/night variation.
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