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At each time point Grade 2 nuclei predominated, followed by Grade 1 nuclei in 3 of 4 evaluations.
The number of TRAP-positive cells with 1 or 2 nuclei (mononuclear osteoclasts) or with 3 or more nuclei (multinuclear osteoclasts) was recorded for each site, and the mean number of multinuclear and mononuclear osteoclasts was calculated per cm.
The cytokinesis-block proliferation index (CBPI) was calculated by the formula: CBPI = (M1 + 2 M2 + 3 M3)/ N, where M1- M2 represents the number of cells with 1-2 nuclei, M3 represents the number of cells with more than 2 nuclei, and N is the total number of scored cells [ 52, 53].
Not all of the BN cells in the abattoir monolayers had p53-positive nuclei (in some cases only one of the 2 nuclei in one cell contained stabilised p53, and some of them were both p53- and p21-negative.
In type 2 nuclei, we define the pseudocases to be the two HLA haplotypes found in the proband and the pseudocontrols to be those found in the spouse.
As premature expression of NDT80 resulted in a significant reduction in the level of cells that entered MII, (most cells accumulated with 2 nuclei, Figure 6C), we suggest that MII inhibition was due to inhibition of Cdk1 activity, most probably due to Swe1 activation.
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It has been suggested that the surface of 81P/Wild 2 nuclei remains very old ancient terrain, presumably dating back to the comet's residence in the trans-Neptunian region (e.g., Brownlee et al., 2004).
Multinucleated (>2 nuclei) TRACP+ cells were counted and scored under microscopy.
rd1 nuclei, which had an elongated form and normal size but displayed a darker euchromatin and sharper heterochromatin borders, were categorized as Stage 1. rd1 nuclei, with a smaller and rounded appearance, and with proportionally much more heterochromatin, with sharp borders were labeled as Stage 2. Some stage 2 nuclei also showed a euchromatin-containing area at their rim.
Results shown in Fig. 6A indicate the distribution of representative subcellular markers along a sucrose gradient (from 10% to 50% sucrose concentration), obtained upon fractionation of Luc-shRNA HK-2 nuclei-free cell extracts.
Since HCA media can utilize variable % polarization and concentration or may involve detection of nuclei other than spin-1/2 nuclei (i.e., H, C, Xe, etc)., we were prompted to implement a new quantity of measure incorporating these variables as described below.
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