Firefly makes a special one with 2 nubs in the bottom to create a better more central spin due to having two contact points on the BB instead of one.
In addition to CNS development [ 3, 11], the nub and pdm-2 genes are also expressed in wing imaginal discs [ 25] and the larval hindgut [ 10], respectively.
Coexpression of BUBR1 Cub URA3 with either Nub:BUB3.1 and Nub MAD2 conferred resistance to 5-FOA, indicating that BUBR1 interacted with both BUB3.1 and MAD2.
For example, the two embryonic NB enhancers for nub and pdm-2, nub-46 and pdm2-34, alsoactivectine in larval NBs (Fig. 8a, c, respectively).
These include those located upstream to the nub long transcript (nub-12 and nub-13), its first intron (nub-28), second exon (nub-32a), second intron (nub-32b, nub-32c, nub-33, nub-36, nub-40b, nub-41, nub-42, nub-44, and nub-45a), and third intron (nub-49b) (Fig. 3).
For example, we detected embryonic enhancer activity for nub-9, nub-11, and nub-12, whereas nub-8, nub-10, and nub-13 did not activate reporter expression during embryogenesis.
These enhancers (nub-19, nub-31, nub-32b, nub-36, and nub-41) were active in different imaginal discs (described in Additional file 4: Table S1 and shown at cisPatterns).
It is worth noting that we identified 4 of the 93 non-SOG CSCs (nub-27, nub-34, nub-49a, and pdm2-23b) clustered within the SOG enhancer group (Fig. 10b, asterisks).
The orange boxes represent the nub-46, nub-49a, pdm2-34, and pdm2-37a enhancers that direct NB expression.
Similar to nub-41, nub-44 also drove expression in a subset of lateral cephalic lobe NBs (Fig. 5c).
