We either crossed the glr-2:: gfp marker described into these backgrounds or created new extrachromosomal glr-2:: gfp arrays by injection into appropriate strains.
However, these animals exhibited a strong up-regulation of Ptbx-2:: gfp expression in the head neurons where Ptbx-2:: gfp is normally expressed.
rgs-2 ::GFP labels uterine muscles in both wild type and lin-12 wy750) lin-12 wy750
The expression of lag-2 :: gfp was quantified in early to mid-L3 stage animals.
ceh-2 :: gfp and daf-6 :: yfp showed a more restricted pattern of expression.
For lag-2 :: gfp expression analysis, two different transgenic lines, qIs56 and arEx1352, were used.
Notably all nfyb-1 (cu13 ) mutants exhibited Ptbx-2:: gfp expression in H0 and the gut.
In addition, occasional Ptbx-2:: gfp expression also was observed in the ventral nerve cord.
Cell permeability was also analyzed in Annexin 2-GFP transfected MDCK cells.
In absence of extracellular Ca2+ no Annexin 2-GFP movement was detected (not shown).
