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ATAD2: ATPase family; AAA: domain containing 2; ESC: embryonic stem cell; HP1: heterochromatin protein 1; iPSC: induced pluripotent stem cell; REST: repressor element silencing transcription factor.
To address this question, we performed ESC-derived EpiSC ESD-EpiSCC) colony formation assays (Zhang et al., 2010a) using day 2 ESC aggregates in serum-free medium.
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These mice were divided into nine groups according to the weight: control (saline), solvent (10%DMSO), 20 mg/kg 5-Fu, 4.5 mg/kg ESC, 3.0 mg/kg ESC, 25 mg/kg ESC-1, 16.75 mg/kg ESC-1, 1.5 mg/kg ESC-2, 1.0 mg/kg ESC-2.
According to the effect strength, dose and antitumor spectrum, the order of antitumor effects of ESCs was: ESC-2 > ESC > ESC-1.
(A) Representative images of outgrowth expanded from NOD-scid Il2rg −/− blastocysts (left), day-2 ESC colonies after single-cell passaging (middle), day-2 ESC colonies after AP staining.
Figure 23 ESC incorporation in ELAN environment.
Figure 21 ESC training for annotation.
Figure 12 ESC qualitative results on GSL using geometrical features.
Figure 13 ESC qualitative results on GSL using LAAMs features.
Figure 11 ESC qualitative results on GSL using GAAM features.
Figure 22 ESC vs. supervised classification with scrambled manual annotations.
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