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Virus titers reached more than 10 TCID50/ml after the 1st passage and remained stable for 12 passages.
The cell-associated virus titer obtained from HmLu cells infected with the 007Lm strain was 2 × 102 PFU/ml at the 1st passage.
The obtained titers of cell-free and cell-associated viruses were 1.3 and 0.6 × 106 PFU/ml, respectively, at the 1st passage.
Following standard procedures, the MEFs (WT and casp2−/−) were cryopreserved after 1st passage.
injected with cattle BSE (1st passage) and a Tg(OvPrP4) mouse infected with classical cattle BSE (2nd passage).
DNA synthesis, as measured by H-thymdine uptake was significantly attenuated in nestin-depleted 1st passage aortic VSMCs (N = 4).
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· 1-5 passage Ruelle, Paris.
Le Passage is at 18 Passage de la Bonne-Graine.
(3) Passage of the American Board of Internal Medicine Institute for clinical evaluation, ECG certifying examination. .
Stage 3: passage through regional intermediaries/international traders.
Progeny viruses from trial 1 (passage 7) and trial 2 (passage 6) were plaque purified.
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