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Every third section was counted for PN7 and PN14 ovaries, every fifth section counted for 3 weeks ovaries, and every 10th section for 3 months and 6 months.
Every 10th section (25 sections out of 250 sections from each cord), spanning ∼4 mm in length across the lesion site (∼2 mm caudal to ∼2 mm rostral to the lesion epicenter), was stained for EGFP expression.
The CA1 region of counting was outlined under 5× objective lens on every 10th section of 35-μm-thick cryostat sections from 0.24 to 1.36 mm lateral from the midline on one hemisphere of the mouse brain, according to the stereotaxic coordinates adopted from a mouse brain atlas (Franklin & Paxinos, 2001).
As described above, the 12-µm sections used for DNA content estimation were selected by systematic random sampling (every 5th section in P3 mice and every 10th section in P30 animals).
Slides containing every 10th section of ipsilateral L3, L4 or L5 DRG of animals sacrificed one or six weeks after collagenase or saline injection were used for immunofluorescence reactions against TRPV1.
Uterine tissues were fixed in Bouin's solution, embedded in paraffin, and serially sectioned every 9 μm; every 10th section was mounted on slides.
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See notes 11, 13, and section 3.4.
Page B16 Section ST.
The worst seat: N2, Section 108.
See remark 2 (section 5).
(See M part 3, section 2).
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