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In stage 2, a subset of promising variants found in stage 1 were further evaluated in an independent cohort of 1001 subjects (254 SAD and 747 controls).
The multi-factor optimization data (Additional file 1) were further analyzed by linear regression with the instrument response as the dependent variable in order to determine the influence of the three univariate components.
The multivariate optimization data (Additional file 1) were further analyzed by linear regression with the instrument signal as the dependent variable, and it was found that the single important factor influencing the peak height is extraction speed.
Hence, the effects of relatively more selective antagonists for the α1-adrenoceptor subtypes (i.e. 5-methylurapidil [α1A], L-765,314 [α1B] and BMY 7378 [α1D]) and the α2-adrenoceptor subtypes (i.e. BRL44408 [α2A], imiloxan [α2B] and JP-1302 [α2C]) (Table 1) were further investigated in an attempt to identify the subtypes involved.
All the successfully purified RNAs (Table 1) were further used in biochemical assays aimed at characterizing their folding, catalytic, and structural properties.
A subset of 41 isolates, including all those described in Table 1, were further confirmed as Aeromonas spp., based on partial 16S rRNA gene sequencing [21].
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In this article, q = 1 is further assumed.
Each cost function in (1) is further explained as follows.
Category 1 is further divided into 1a 1f subcategories.
Cluster 1 was further subdivided into two sub-clusters.
The 13 were further divided into 2 A growths and 11 B growths.
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