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The proposed purification protocol (see Figure 1, underlined methods) provides good yield, purity, coupling efficiency and preservation of different size transcripts.
A SphI site was included in the primer 1 (underlined) and a XhoI site was included in primer 2 (underlined).
Clade 1 (underlined in yellow in Figure 6) includes exclusively 8 S.
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c) Integration site sequences retrieved from TAIL-PCR analysis of transformants #2, #8, #10, #11 and #12; The underlined and highlighted in bold sequences represent the portion of the T-DNA adjacent to the unknown sequence obtained by PCR.
(Chapter 4, underlined: "I remember Mr. Hubble as a tough high-shouldered stooping old man, of a sawdusty fragrance, with his legs extraordinarily wide apart: so that in my short days I always saw some miles of open country between them when I met him coming up the lane").
The authors consider two sub-scales: psychic anxiety (items 1 6 and 14) and somatic anxiety (items 7 13, underlined supra).
Table 7 The accuracies of activity and identity recognitions through the recommended combination from VISEE Type RF activity RF identity LMT identity MP identity Accuracy 95.1765 32.4316 29.6331 29.6331 Underlined values indicate the best accuracy result achieved by a certain model among all available models.
Table 6 The accuracies of activity and identity recognitions based on feature set (F_6) Type RF activity RF identity LMT identity MP identity Accuracy 96.646 59.638 64.203 61.159 Underlined values indicate the best accuracy result achieved by a certain model among all available models.
GC = guanine-cytosine content; aa = amino acids, = values in brackets are for the 1485 bp long gene fragment of ORF2; underlined sequence parts = putative Shine-Dalgarno sequence (RBS), sequence in boldface = assumed start codon, sequence in italics = potential alternative start codon.
The sequence at the junction between KIR2DL1 and the CD4 was GNPRHLHGSTSITAYK (KIR2DL1 underlined).
The primers included new restriction sites, SacI at the 5'end and NheI at the 3'end (underlined bases).
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