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Higher cumulative uranium fluxes (as seen with concentrations > 1 ug U/gram resin) yielded more homogeneous resin samples versus lower cumulative fluxes (<1 ug U/gram resin), which caused the PFM to have areas of localized concentration of uranium.
Although the inhibitory effect of BNP was increased from 1 ug to 2 ug, but was almost unchanged when the dose was further increased to 3 ug.
Complementary (c) DNA was synthesized by using 1 ug of total RNA, Moloney Murine Leukemia Virus Reverse Transcriptase, and polyT primer as described previously (Chen et al. 2008).
cDNA was prepared using Superscript polymerase (Invitrogen) using 1 ug RNA.
For BiFC, Anx2-FR1 and p41Gag-FR2 were co-transfected in the ratio of 1 ug Anx2 or CD63: 2 ug p41Gag or Gag along with 1 ug empty vector, 5ptase IV, or Arf6/Q67L.
Briefly, 1 ug of biotinylated peptides were incubated with 1 ug of GST-domain in peptide binding buffer (50 mM Tris-HCL, pH 7.5, 150 mM NaCl, 0.1% NonidetP-40) overnight at 4°C.
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*DEG was filtered using threshold of false discovery rate (FDR) ≤ 0.001 and absolute value of log2Ratio ≥ 1. Ug represents unigene.
The detection range using this nanoplasmonic immunoassay ranges from 10 ng/mL to 1 ug/mL for Glut-1.
DNA was visualized with Propidium Iodide (1 ug/ml) or DAPI (1 µg/ml).
Stable lines were selected over 2 3 weeks by incubation in medium containing 1 ug/ml puromycin.
Data from the initial experiments informed us that 5 ug/ml C4BP was the minimum concentration required to completely inhibit apoptosis induced by 1 ug/ml sCD154.
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