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Five of the 17 patients (4, AV canal; 1, transposition) underwent valve replacement.
In 12 of the 17 patients (10, AV canal; 1, transposition; 1, hypoplastic left heart), valve reconstruction was possible.
More interesting are clusters with high diversity with respect to species, but low diversity with respect to function, e.g. clusters 1 (transposition), 5 (photosynthesis), 6 (regulation of transcription), 11 (flagellum), and 12 (locomotion, chemotaxis).
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This is consistent with metabolism being the most affected biological function (19%), followed by secretion (14%), protein folding (6%), transcription (6%), transposition (6%), and physiological defense response (4%).
Simons, R.W. & Kleckner, N. Translational control of IS10 transposition.
The Tn7 transposition events were then mapped using two complementary assays.
Roberts, D., Hoopes, B. C., McClure, W. R. & Kleckner, N. IS10 transposition is regulated by DNA adenine methylation.
In this project, an in vivo Tn7 transposition assay was used to investigate the role of the SeqA-- TnsE interaction in the TnSeqA-- TnsEof Tn7 tranSeqA-- TnsE
Kleckner N, Chalmers RM, Kwon D, Sakai J, Bolland S. Tn10 and IS10 transposition and chromosome rearrangements: mechanism and regulation in vivo and in vitro.
Four basic cardiac anomalies accounted for 75% patients: one ventricle (26), atrial septal defect (ASD) (19), transposition of great arteries (TGA) (17) and Tetralogy of Fallot (13).
Quantification of the relative amounts of the fluorescent Tns proteins in the PTC indicates that oligomers of TnsA, TnsB, and TnsC mediate Tn7 transposition.
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