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31 The trial outcome was not modified when less (that is, seroconversion based on a 1 titre difference) or more (that is, higher direct agglutination test cut offs) specific criteria were used (see appendix on bmj.com).
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In most seroreactive A goats (Fig. 1), titres rose from about week 3 p.i., similar to previous findings [ 18, 19].
All laboratories measured lower anti-CHAT than anti-Sabin 1 titres in both their internal reference sera and patient sera.
It was noticeable that within the subset groups (α and M), α1 shows significantly higher (p = 0.0068) titre compared to α2, despite having lower copy number of the heavy chain (Additional file 2: Figure S2).
As expected, the S1P titre was found to be elevated in the infected SphK-1++ macrophages.
Results are expressed as the log2 titre at which no haemagglutination was observed.
Four fold or greater reduction of H5 titre in treated sera indicated cross-reactivity.
The TCID50 titre was calculated using the Reed & Muench method [39] and normalized per gram of lung tissue.
The GFP expression was validated by FACS, and SphK-1 overexpression was confirmed by western blot, immunofluorescence staining and competitive S1P titre (Fig. 2A).
Serum samples showed statistically indistinguishable gp140-specific IgG1 responses (Figure 4B), but significantly reduced IgG2a titres (Figure 4C, p = 0.0181, unpaired t-test) and significantly increased IgG1/IgG2a titre ratios (Figure 4D, p = 0.0379, Mann Whitney test) for animals receiving gp140 + PRO 2000 in FCA.
The IgG1 titre was significantly lower in Hg-treated FcRγ−/− mice than in Hg-treated wt mice (Figure 4D) (p<0.05) (Table 2).
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