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The 96-h didactic program focused on core EM competencies and included lectures (76 h; Table 1) supplemented by a variety of other teaching modalities.
salicylic acid, abscisic acid, phenylalanine, sodium acetate and jasmonic acid (at a fixed concentration 3 mgL − 1) supplemented individually to 1/2X MSBM.
Dataset 3 consists of the Swedish individuals of Dataset 1 supplemented by Swedish cases (774 cases and 751 controls).
Supernatants were decanted and the nuclear pellet was washed twice with buffer 1 supplemented with 0.1% NP-40 (wash buffer), first employing 5 ml wash buffer/g embryo and next with 2 ml wash buffer/g embryo.
The cell lines were cultured in HAM/F12 and DMEM medium (1 : 1) supplemented with 20% FCS.
The HSF cell line was established from skin explant and maintained in DMEM/MEM (1 : 1) supplemented with 10% FCS.
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Increasing GAG sulfation improved equine tenocyte metabolic activity in normal serum (10% FBS), low serum (1% FBS), and IGF-1 supplemented media conditions.
BT-474 cells were cultured in DMEM F-12 (1:1) supplementeDMEM F-12% fetal bovine supplementedl-glutamine, nonessential amino acids, penicillin, and streptomycin.
The culture medium was Williams and HAMs F-12 (1 : 1) supplemented with penicillin, streptomycin, ascorbic acid, linoleic acid, holo-transferin, ethanolamine, glucagon, insulin, dexamethasone, pyruvate, glucose, glutamine and amphotericin.
Following incubation, the cells were washed twice to remove unbound biotinylated antibodies with 2 mL of labelling buffer composed of PBS (1×), supplemented with 2 mM EDTA, and centrifuged.
Cells were grown in DMEM F-12 meDMEM F-12 supplemediad with FBS (1 1%) and 100 U/mL penicillin and 100 μg/mL supplemented in 150 cm2 culture flasks in a humidified atmosphere of 5 % CO2.
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