Exact(6)
At 48 h after the transfection, CM were harvested for precipitation with Ni NTA agarose (1 : 1 slurry; Invitrogen) or anti-FLAG antibody M2- conjugated agarose (1 : 1 slurry; Sigma-Aldrich).
Immune complexes were precipitated with 30 µl 1 : 1 slurry Protein-A sepharose (Invitrogen) for 30 min at 4°C.
Insoluble debris was pelleted and lysates were pre-cleared with a 1 : 1 slurry of sepharose in lysis buffer for 1 h at 4 °C with constant agitation.
One hundred microliters of purified plasma was coincubated with 20 μl of 1 : 1 slurry of GST-CLSP antibody or preimmune-conjugated beads at 4 °C overnight for immunoprecipitation.
For co-immunoprecipitation experiments, protein extracts (1 mg) were precleared and incubated overnight with a specific CD9 mAb (2 μg of MM2/57) and protein Sepharose G beads (1 : 1 slurry, GE Healthcare, Baie d'Urfe, QC, Canada).
For immunoprecipitation, supernatants were mixed with an antibody at 4 °C for 2 h and then mixed with 1 : 1 slurry of protein G-sepharose 4B and rotated overnight before washing with Buffer1.
Similar(54)
The limit of detection of the method was 0.02 μg g−1 (100 mg mL−1 slurry), and the characteristic mass was 15 ± 1 pg.
SRB from all four targeted SRB groups were detected in underground waste storage pits (6.6×103 8.5×107 dsrA copies mL−1 slurry), while only two groups of SRB were detected in lagoons (3.2×105 2.5×106 dsrA copies mL−1 slurry).
For TiO2 layer prepared from solutions containing less than 20 wt.% of TiO2 slurry, the thickness of layer was increased linearly with the TiO2 slurry content in solutions.
TiO2 films were prepared by doctor blading of TiO2 nanoparticle (P25) slurry on FTO/glass substrates.
The photoreactor system of about 364 containsains 1.2 L of pure methanol and a TiO2 slurry of 1 g/L.
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