We found that JA did not affect DR5:uidA and BA3:uidA expression in WT and coi1-1 seedlings.
Total RNA was extracted with the RNAeasy Plant Minikit (Qiagen, http://www.qiagen.com/) from WT, bet11-1 and bet12-1 seedlings.
The root growth rate of the cpr5-1 seedlings was less than that of the wild-type plants and the sensitivity could be reversed by CPR5 overexpression (Figure 3H).
As was seen for wild-type seedlings, hypocotyls of the SOFL1-RNAi62 sofl2-1 seedlings were shorter in the presence of cytokinin, but a reduced response was observed when compared to the wild type (P<0.05, Figure 6).
To corroborate the notion that SUB receptors can be subject to ERQC/ERAD we treated wild-type or sub-1 seedlings carrying the sub-3 and sub-10 mutations in the ECD of SUB EGFP with kifunensine (Kif).
Unlike on soil, fln1-1 seedlingrownonn on media supplemented with 1% sucrose produce true leaves.
Gene expression in hsr8-1 and soh175hsr8-1 seedlings was assessed using the ATH1 Gene Chip.
EBR-treated aba1-1 seedlings accumulated approximately 3- and 2.5-fold higher levels of hsp90 at 3 and 4 h of HS, respectively, as compared to untreated aba1-1 seedlings.
Similarly, an evident decrease in pri-MIR156C was observed in the gin2-1 seedlings treated with ActD/glucose.
