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Figure 1 SDS (A) and Native (B) PAGE for Laccase produced by P. ostreatus IBL-02.
Figure 1 SDS- PAGE ( 15%) analysis of ASNase II purification using DEAE-Sepharose.
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Based on the increment of IGF-1 used during treatment, patients were divided into 2 groups: G1 (>1 SDS) and G2 (<1 SDS).
Lysates were then prepared in 1× SDS sample buffer.
Cells were washed twice with PBS and lysed in 1× SDS sample buffer.
Protein precipitates were recovered by centrifugation, washed with acetone and dissolved in 1× SDS sample buffer.
For analysis by Western blotting, mitochondria were pelleted and resuspended in 1× SDS sample buffer + DTT.
The patient was born at term with a birth weight of 3040 g (−1 SDS).
Membranes were then washed three times in 2 × SSC buffer with 1% SDS, and twice in 0.6 × SSC with 1% SDS for 30 min at 68°C.
To solubilize the films, the samples were mixed with a solubilizing solution (1% SDS).
The untreated nanoparticles (without boiling in 1% SDS solution) were kept as control.
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