Sentence examples for 1 rnp from inspiring English sources

We purified cytosolic RNP after 1 h of transfection (Type 1 RNP) by immunoselection with anti-RIC4A antibody.

Since rnp-1 mRNA is present throughout the germ line, but the OMA proteins are first expressed in late pachytene (Detwiler et al. 2001; Figure 1), RNP-1 is likely expressed earlier in germline development.

What other proteins are parts of the Gadd45 RNP particle?

However, in the bI3 RNP, every long-range RNA interaction is stabilized by a bound protein cofactor.

These observations suggest that the RNA largely plays a passive role within the L1 RNP as a retrotransposition intermediate.

However, SAMHD1 reduced ORF2p expression and suppressed ORF2p-mediated LINE-1 reverse transcription in purified LINE-1 RNP.

Notably, our OMA-1 RNP purifications identified all the previously known and newly identified OMA-1-interacting proteins save for three exceptions: TAF-4, C27B7.2, and DH11.5.

Further these results indicate that the change in OMA-1 RNP localization in the absence of MSP-dependent signaling is independent of its translational repression activity.

Mass spectrometry is extremely sensitive, and >250 different proteins were identified by at least two peptides in both OMA-1 RNP purifications.

Also, LINE-1 RNA was extracted from the LINE-1 RNP, treated with TURBO DNase (Invitrogen), and reverse transcribed using 3′ RACE adaptor NV as primer and MuLV RT with a High Capacity cDNA Reverse Transcription Kit (ABI Applied Biosystems).

The LINE-1 RNP sample (2 μl) was added to each cDNA extension reaction (LEAP) as previously described (Kulpa and Moran, 2006), using the 3′ RACE adaptor NV: 5′-GCGAGCACAGAATTAATACGACTCACTATAGG TTTTTTTTTTTTVN-3′ as primer.