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The NYU RIPS collaboration, led to a co-publication between 2 core working group members, 4 NYU MPH students and 1 RIPS MPhil student [ 27].
Saporins belong to a multigene family of single chain (type 1) RIPs, which are formed in various organs of Saponaria officinalis (soapwort).
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Cap 1: RIP to what? 2 Chainz: RIP this stage.
For PUM2 RIPs, we performed four biological replicates but omitted the dye swaps due to the lower aaRNA obtained after amplification (∼10 µg aaRNA from PUM2 RIPs, ∼40 µg aaRNA from PUM1 RIPs, ∼9 µg aaRNA from mock RIPs).
For PUM1 RIPs, we performed three biological replicates with technical (dye swap) replicates (total six arrays).
Fold enrichment of transcripts from the anti-FLAG MKRN1 anti-FLAG MKRN1ated RIPstive to the twascalculatedPs: anti-FLAG FLAG:Ctrelativend anto-IgG1 FLAG:MKRN1 RIP.
Similarly, 37 marker loci were shared between sets of 3 RIPs, 52 marker loci were shared between pairs of RIPs, and 86 marker loci were unique to one or the other of the four RIPs.
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